Sexing frogs can be a difficult task, even for the most experienced breeders, but there are some distinct signs you can try to identify. It is best to wait until the frog reaches sexual maturity to identify the frog’s sex. Some poison arrow frogs take six months to sexually mature, while others can take up to two years. Males are usually smaller and thinner than females. Males also have larger toe pads due to the fact that they transport their young on their backs to areas of substantial standing water, although this is not true with every species. Males call while females do not (males have vocal sacs, which females lack), and males guard egg-laying sites. Potential breeders need to be aware that first clutches of eggs are often infertile.
Some frogs will breed year-round, but I recommend providing your frogs with seasonal variations. It is important for the frogs’ health to give them a break for a few months out of the year. This is best achieved by lowering the ambient temperature in the cage by 3-4 degrees and reducing the humidity by 10-15 percent. If you are not breeding your frogs, obviously this is not relevant to their general health.